Isolasi Jamur Endofit dari Sonneratia Alba dan Toksisitasnya Terhadap Artemia Salina
DOI:
https://doi.org/10.32670/ht.v1i7.1707Keywords:
Sonneratia alba, Endophytic fungi, Brine Shrimp Lethality TestAbstract
Endophytic fungi are microorganisms that live in plant tissues
asymptomatically. Endophytic fungi can produce secondary metabolites with
various biological activities, one of which is anticancer. Mangroves are known
to be a potential source of endophytic fungi that have active secondary
metabolites. One of the dominant species of mangrove is perepat (Sonneratia
alba). This study aims to isolate endophytic fungi, isolate and identify
endophytic fungi from adhesives (S. alba), as well as toxicity test using the BSLT
method on Artemia salina shrimp larvae. The study began with the isolation and
purification of endophytic fungi, macroscopic and microscopic identification,
cultivation and extraction, and toxicity test using the Brine Shrimp Lethality
Test method. The isolation results of the leaf endophytic fungus S. alba were
isolate SA-L1-1 which after being identified macroscopically and
microscopically had similar characteristics with Nigrospora sp. In the toxicity
test, the endophytic fungal isolate extract SA-L1-1 was toxic to A. salina shrimp
larvae with an LC50 of 405.109 ppm.
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